Isolation and Identification of Mycobacterium Tuberculosis: A Guide for the Level II Laboratory

100 ml distilled acid-fast bacilli Acid-Fast Stain aerosol agar medium Amer amphyl aseptically auramine autoclave bacterial suspension biological safety cabinet buffer Bunsen burner catalase clean Clin clinical specimens color concentrated condenser container culture decontamination described digested dilution discard discs dish disinfectant Dissolve distilled water drops drug medium drug susceptibility test egg medium equipment ethanol Figure filter Flood smear fluid fluorescence microscopy fluorochrome fuchsin gastric lavage growth identification incubation inoculated inoculum isolation room Kubica laboratory light loop material method microscope ml distilled water mycobacteria mycobacteriology negative niacin test Nitrate reduction test oil immersion objective organisms patients personnel phosphate pipette pipettor Place plastic plates positive potassium permanganate prepared procedures quadrants remove Respir saline screwcap screwcap test tubes sediment slant slide sodium Sodium hydroxide solution spade species sputum test tube mixer tion tubercle bacilli tuberculosis volume water bath